Improvement of Invertase Synthesis by the Mutant Saccharomyces cerevisiae through UV Mutagenesis
نویسندگان
چکیده
منابع مشابه
Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase.
Efficient enzymatic synthesis of tailor-made prebiotic fructo-oligosaccharides (FOS) used in functional food formulation is a relevant biotechnological objective. We have engineered the Saccharomyces cerevisiae invertase (Suc2) to improve its transferase activity and to identify the enzymatic determinants for product specificity. Amino acid replacement (W19Y, N21S, N24S) within a conserved moti...
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Present investigation deals with optimization of appropriate substrate concentration and incubation temperature both for growth of Saccharomyces cerevisiae and invertase production. Submerged fermentation technique was employed in the present study. The maximal production of invertase during the course of study was achieved after 48 h of incubation using initial sucrose concentration, 15.0 g l....
متن کاملRegulation of invertase synthesis by glucose in Saccharomyces cerevisiae.
Saccharomyces cerevisiae growing under repressible conditions (1% of glucose or more) produces a burst of external invertase when shifted to higher temperatures. The secretion of this invertase requires protein synthesis, but was found to be independent of RNA formation. The level of mRNA accumulated and translated was inversely proportional to the glucose present in the growth medium. These re...
متن کاملEfficient maltotriose fermentation through hydrolysis mediated by the intracellular invertase of Saccharomyces cerevisiae
Background It is well known that in the yeast S. cerevisiae the sugars sucrose and maltose/maltotriose are metabolized by different pathways: sucrose is hydrolyzed by extracellular invertase (encoded by SUC genes), while maltose and maltotriose are actively transported into the cell and hydrolyzed by intracellular a-glucosidases (both proteins encoded by the MAL genes). Nevertheless, several re...
متن کاملKinetics of Invertase Production by Saccharomyces Cerevisiae in Batch Culture
The present study is concerned with the improved invertase production by Saccharomyces cerevisiae on kinetic basis. The wild-culture (IIB-1) and putative mutant (NA6) of S. cerevisiae were compared on time course basis when grown in batch culture. The values for Yx/s, Yp/s and Yp/x with urea were significantly improved (p≤0.05) over the control (peptone+yeast extract) as well as peptone supplem...
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ژورنال
عنوان ژورنال: Universal Journal of Microbiology Research
سال: 2017
ISSN: 2331-6721,2331-673X
DOI: 10.13189/ujmr.2017.050201